Perfluorocarbon restrains inflammation and cell apoptosis in rats with lung ischemia reperfusion injury via down regulation of TLR4/NF κB signaling pathway

Purpose: To exaluate the effect of perfluorocarbon on lung ischemia-reperfusion injury in rats, and to unravel potential underlying mechanism.Methods: A total 36 Sprague-Dawley (SD) rats were randomly assigned sham group, model group (12 per group). The levels inflammatory factors (TNF-α IL1β) determined using enzyme-linked immunosorbent assay (ELISA). Cell apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, while Western blotting conducted determine protein expressions TLR4 NF-κB.Results: groups significantly higher than those operation (p < 0.05), their lower 0.05). mRNA expression TNF-α IL-1β tissues rose both when compared with but declined comparison Furthermore, exhibited a cell relative NF-κBdeclined group.Conclusions: Perfluorocarbon down-regulates TLR4/NF-κB signaling pathway, inhibits inflammation an